Journal: Lasers in Medical Science

Article Title: Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

doi: 10.1007/s10103-023-03885-0

Figure Lengend Snippet: Gene sequence

Article Snippet: The primary antibodies were listed in the following manner: GAPDH (ABclonal,1:5000,USA), NFATC1 (ABclonal,1:1000,USA), TIMP-1 (ABclonal,1:1000,USA),TIMP-2 (ABclonal,1:1000,USA), MMP-1 (ABclonal,1:1000,USA),MMP-2 (ABclonal,1:1000,USA), Runx2 (ABclonal,1:1000,USA),MMP-9 (ABclonal,1:1000,USA), COL-I (ABclonal,1:1000,USA), COL-III (ABclonal,1:1000,USA), c-Fos (CST, 1:1000, US), OCN (CST, 1:1000), CTSK (Abcam,1:1000).

Techniques: Sequencing

Journal: Lasers in Medical Science

Article Title: Effects of low level laser on periodontal tissue remodeling in hPDLCs under tensile stress

doi: 10.1007/s10103-023-03885-0

Figure Lengend Snippet: Collagen-modified factors detection ( a ) Quantitative real-time PCR analysis of Col-I and Col-III mRNA expression of hPDLCs. n = 3 . ( b ) Western blot analysis of Col-I and Col-III protein levels of hPDLCs. n = 3 . Data represent the mean ± SD of three independent experiments. *P < 0.05 vs. the control group, #P < 0.05 vs. the tensile stress group and &P < 0.05 vs. the laser group by one-way ANOVA

Article Snippet: The primary antibodies were listed in the following manner: GAPDH (ABclonal,1:5000,USA), NFATC1 (ABclonal,1:1000,USA), TIMP-1 (ABclonal,1:1000,USA),TIMP-2 (ABclonal,1:1000,USA), MMP-1 (ABclonal,1:1000,USA),MMP-2 (ABclonal,1:1000,USA), Runx2 (ABclonal,1:1000,USA),MMP-9 (ABclonal,1:1000,USA), COL-I (ABclonal,1:1000,USA), COL-III (ABclonal,1:1000,USA), c-Fos (CST, 1:1000, US), OCN (CST, 1:1000), CTSK (Abcam,1:1000).

Techniques: Modification, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Control

Journal: Parasites & Vectors

Article Title: MicroRNA-497 induced by Clonorchis sinensis enhances the TGF-β/Smad signaling pathway to promote hepatic fibrosis by targeting Smad7

doi: 10.1186/s13071-021-04972-3

Figure Lengend Snippet: Inhibition of miR-497 in mice reduced CCl 4 -induced liver fibrosis. a Collagen deposition shown by Masson staining in the livers from different groups as indicated. b Sirius red staining showed collagen deposition in the livers from different groups as indicated. c Hydroxyproline (HYP) content in the liver of mice in each group as indicated. d Levels of α-SMA, P-Smad2/3, COLI, and COLIII in in the livers from different groups determined by western blot. * P < 0.05, ** P < 0.01, compared with the indicated group. All data represent at least three independent experiments

Article Snippet: The membranes were cut into bands of appropriate width according to the protein marker and after 60 min of blocking with 5% fat-free milk, membranes were incubated with Smad 7 antibody (1:2000; Sigma, USA), COLI antibody (1:2000; Sigma, USA), P-Smad2/3 antibody (1:1000; Abclonal, Wuhan, China), COLIII antibody (1:1000, Abclonal, Wuhan, China), α-SMA (1:1000, Abclonal, Wuhan, China), and β-actin antibody (1:2000; Abmart, Shanghai, China) overnight at 4°C, correspondingly.

Techniques: Inhibition, Staining, Western Blot